Journal: bioRxiv

Article Title: Pufferfish ( Tetraodon cutcutia ) sampled from freshwater river serves an intermediate reservoir of sucrose nonfermenting variant of Vibrio cholerae PS-4

doi: 10.1101/2021.10.27.466062

Figure Lengend Snippet: Growth response of V. cholerae strain N16961 and V. cholerae strain PS-4 on TCBS (Thiosulfate-citrate-bile salts-sucrose agar) and Luria Bertani agar supplemented with 0.5% glucose or 0.5% fructose and 2.0 mg/L bromothymol blue. V. cholerae N16961: A1, growth on TCBS; A2, growth on Luria Bertani agar plus glucose; A3, growth on Luria Bertani agar plus fructose. V. cholerae PS-4: B1, growth on TCBS; B2, growth on Luria Bertani agar plus glucose; B3, growth on Luria Bertani agar plus fructose.

Article Snippet: Utilization of sugars were tested separately by adding 0.5% concentration of glucose or fructose in Luria Bertani Agar medium (BD, Difco) containing bromothymol blue 2.0 mg /L as pH indicator, at 37 °C for 48 h. To ascertain the haemolytic activity, strain PS-4 was streaked on Columbia blood agar base supplemented with 5% (v/v) defibrinated sheep blood followed by incubation at 37 °C for 48 h ( ).

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